FERMENTASI PAKAN OLEH MIKROBA RUMEN DALAM SISTIM CONSECUTIVE BATCH CULTURE (CBC)
DOI:
https://doi.org/10.20956/bnmt.v9i1.849Abstract
Pola dan trend degradasi pakan oleh mikroba rumen bervariasi antara bahan pakan satu dengan bahan pakan lainnya. Slope (koefisien regresi) bahan kering tercerna kulit buah coklat, jonga-jonga, dan biji markisa masing-masing adalah –0.62, -0.08, dan –2.34. Sedangkan slope bahan kering tercerna untuk biji kapuk dan kulit markisa adalah 0.20 dan 2.23 menunjukkan bahwa mikroba rumen dapat beradaptasi dengan adanya substrat biji kapuk dan kulit markisa dalam medium. Sebaliknya kulit buah coklat, jonga-jonga, dan biji markisa disamping slope-nya negatif, kecernaan bahan kering-nya juga cenderung menurun selama 6 seri masa inkubasi. Kesimpulannya bahwa kecernaan bahan kering in vitro pakan oleh mikroba rumen bervariasi mulai dari 31% untuk biji markisa sampai dengan 48.2% untuk jonga-jonga. Mikroba rumen menunjukkan pola adaptasi selama 6 seri inkubasi terhadap substrat biji kapuk dan kulit markisa yang mengandung lemak, tannin dan gossypol untuk biji kapuk dan tannin untuk kulit markisa.Downloads
References
A.O.A.C. 1984. Official methods of analysis. 12th edition. Association of Official
Analytical Chemists. Washington, DC.
Bryant, M.P. 1972. Commentary on the Hungate technique for cultivation on
anaerobic bacteria. Am. J. Clin. Nutr., 25:1324-1328.
Hobson, P.N. 1969. Rumen bacteria. In: Methods in microbiology. Vol.3B. (Eds. J.R.
Norris and D.W. Ribbons). Academic Press, London and New York. pp. 133-
Hungate, R.E. and R.J. Stack. 1982. Phenylpropanoic acid: growth factor for
Ruminococcus albus. Appl. Environ. Microbiol., 44: 79-83.
Goering, H.K. and P.J.Van Soest. 1970. Forage fibre analysis. USDA Agric.
Handbook No.379, Washington.
Ismartoyo, C.S. Stewart, W.J. Shand and T. Acamovic. 1994. The effect of gossypol
on the rumen protozoal degradation of grass hay (GH) in vitro. Proceedings
of the Society of Nutrition Physiology , 25-30 September 1994, Willingen,
Germany. p. 205.
Ismartoyo, T. Acamovic, and C.S. Stewart.1993. The effect of gossypol on the rumen
microbial degradation of grass hay (GH) under consecutive batch culture
(CBC). Animal Production, 56: 462 (A).
Ismartoyo, C.S. Stewart and T. Acamovic. 1994. In vitro rumen microbial
degradation of a selection oilseeds and legume seeds under consecutive batch
culture (CBC). Animal Production, 58: 453 (A).
Ismartoyo, C.S. Stewart, T. Acamovic and A.J. Richardson. 1995. The effect of
gossypol on the rumen fungal attachment to and degradation of cellulose in
culture. Animal Production, 60: 552 (A).
Ismartoyo dan Budiman, N.2000. A consecutive batch culture system is a new and an
appropriate concept of feed evaluation system in South Sulawesi. Final report
DCRG 2000/2001.
Jouany, J.P. and K. Ushida. 1994. Plant cell-wall degradation by rumen protozoa. In:
Micro-organism in ruminant nutrition (Eds. R.A. Prins and C.S. Stewart, 1994). Nottingham, University Press.pp.69-95.
Orpin, C.G. 1983. The role of ciliate protozoa and fungi in the rumen digestion of
plant cell walls. Anim. Feed Sci. Technol., 10: 121-143.
Steel, R.G.D. and J.H. Torrie. 1980. Principle and procedures of statistics. A
Biometrical Approach. Second Edition. McGraw Hill International Book
Company. Japan. pp.195-229.
Stewart, C.S., M.Fevre and R.A. Prins. 1995. Factors affecting fermentation and
polymer degradation by anaerobic fungi and the potential for manipulation of
rumen function. In: Ruminant Physiology: Digestion, Metabolism, Growth and Reproduction. Proceedings of the eighth International Symposium on Ruminant Physiology. (Eds. W.v. Engelhardt, S. Leonhard-Marek, G.Breves and D.Giesecke). pp. 251-265.
Theodorou, M.K., D.J. Gassoyne, D.E. Akin and R.D. Hantley.1987. Effect of
phenolic acids and phenolics from plant cell walls on rumen like fermentation
in consecutive batch culture. Appl. Environ. Microbiol., 53:1046-1050.
Theodorou, M.K., D.E. Beever, M.J. Haines, and A. Brooks. 1990. The effect of a
fungal probiotic on intake and performance of early weaned calves. Anim.
Prod., 50: 577 (A).